Contact an Account ManagerDevelopment of a chromatographic method for the quantitative determination of minor ribonucleosides in physiological fluids
Summary
Type: HPLC
Application #: 107150
Catalog#: 720002.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x125mm
Detection: UV, 259nm
Conditions: eluent A: 0.2mol/l ammonium formate pH 3.5, eluent B: methanol, 5min isocratic 100% A, 6min A/B 96 + 4 (v/v), then gradient to A/B = 70 + 30 (v/v) in 18min
flow rate 1.3ml/min
Authors: Hagemeier E
Source: J Clin Chem Clin Biochem 22 (1984) 175 - 184
Details
Legend: Fig. 4. Separation of ribonucleosides in physiological fluids, a) synthetic
mixture, 100µl, 2 - 10nmol each, b) 100µl water fraction obtained in the LC
prefractionation step of urine, nucleosides 8,